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Figure 5 | BMC Molecular Biology

Figure 5

From: Alternative splicing of human peroxisome proliferator-activated receptor delta (PPARdelta):effects on translation efficiency and trans-activation ability

Figure 5

PPARδ isoform-specific variability among 5'-UTRs. 5'-RACE products enriched in either full-length PPARδ (PPARδ1) or PPARδ2 obtained using cDNA from placenta (Pl) and adipose tissue (A) according to "Methods" were used as templates for PCR reactions with untranslated exon/exon primer pairs, and with primers that amplify over exons in the coding region as control. A. Exon 4 to 8 (769 bp) amplified using Ex4Fw and Ex8Rev; B. Exon 1 + 2 (139 bp) amplified using Ex1:2Fw and Ex2Rev; C. Exon 1 + 4 (255 bp) amplified using Ex1:2Fw and Ex4:2Rev; D, Exon 3 + 4 (103 bp) amplified using Ex3Fw and Ex4:2Rev; E, Exon 2 + 2a (565 bp) amplified using Ex2Fw and Ex2a:1Rev;F, Exon 2c + 4 (399 bp) amplified using Ex2cFw and Ex4:1Rev;G, Exon 2e + 4 (326 bp) amplified using Ex2eFw and Ex4:2Rev. The sizes of the major bands obtained are indicated and the identities of the products were confirmed by sequencing.

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