Figure 4

Nucleolin depletion activates apoptotic pathways. A. TUNEL staining. HeLa cells transfected with control siRNA # 1 or the nucleolin siRNA mix #2 and #4 were subjected to TUNEL staining 4 days after transfection. B. Quantification of TUNEL-positive cells by counting at least 400 cells in two independent experiments. Error bars indicate ± SD. C. Western blots. At indicated times, protein extracts were analyzed by Western blotting with anti-nucleolin and anti-p53 antibodies. Normalization was performed with tubulin. D. Immunofluorescence of p53 of control or anti-nucleolin siRNA transfected cells 4 days post-transfection. E. Quantitative RT-PCR. Primers to β-actin and p53 were used. Levels of p53 mRNA were calculated against the level of β-actin mRNA as reference gene.