Figure 1
From: An improved genetic system for detection and analysis of protein nuclear import signals
Schematic of the construction of pNIA derivatives used in this study. Restriction enzyme sites used for each cloning step, relevant markers and features of each plasmid are indicated. The construction scheme is detailed in the Materials and Methods section. The sequence of the HA tag and the unique restriction enzyme sites in the polylinker of pNIA-CEN-MBP plasmid is shown at the bottom right. * indicates site that is destroyed