Figure 9
TNTase activity of JEV NS5. The 83-nt RNA was used as a template for RdRp and TNTase activity assays. For TNTase activity assays, reactions were performed in the presence of cold UTP and [α-32P] UTP (lane 3), or in the presence of single [α-32P] UTP (lane 4). An RNA product from the standard RdRp reaction mixture is shown as a control (lane 2). Lane 1, 5'-end labeled 83-nt RNA size marker.