Figure 6

Nuclease S1 treatment of the RNA products synthesized from the 83-nt RNA template. Heat-denatured (+Δ) or untreated (-Δ) RdRp products synthesized from the 83-nt RNA, which represents the 3'-terminal region of JEV genome, were left untreated (-) or digested with nuclease S1 (S1), and resolved on a denaturing polyacrylamide gel. Nuclease S1 treatments were performed in 50 mM NaCl (L; low salt) or 500 mM NaCl (H; high salt). Arrowhead indicates the position of the 83-nt RNA template.