Figure 5
De novo initiation of RNA synthesis from the plus- and minus-strand 3'-UTR of the JEV genome. RdRp assays were performed with the purified wild-type NS5 (WT) and mutant NS5D668A (Mt) in the presence (+) or absence (-) of JEV 3'(+)UTR RNA (A and C), 3' (-)UTR RNA (B and C), and the 83-nt RNA (D) template. RdRp products were analyzed as in Figure 4 by autoradiography. Arrowheads indicate the template positions visualized by ethidium-bromide staining of the gels.