Figure 6
From: Vertebrate DM domain proteins bind similar DNA sequences and can heterodimerize on DNA
Heterodimer formation between DMRT1 and DMRT3. Gel mobility shifts using labeled DMRT1 consensus probe DNA and indicated in vitro translated proteins. Positions of monomeric, and homo- and heterodimeric complexes with DNA are indicated (maroon triangles indicate DMRT1 and gold triangles indicate DMRT3). DMRT3 protein is tagged with a myc epitope recognized by 9E10 monoclonal antibody. As shown in first two lanes, 9E10 does not affect complex formed by DMRT1, and anti-DMRT1 antibody does not affect complex formed by tagged DMRT3.