Strategy to identify glucose and cAMP responsive IEGs and their downstream targets. A) IEGs (immediate-early genes) are genes which are transcriptionaly inducible in a protein synthesis independent manner. IEG products regulate in turn the transcription of downstream targets. Cycloheximide (CHX), a protein synthesis inhibitor, blocks IEG product synthesis and subsequent activation of target transcription. B) Genes induced by glucose and cAMP comprise both IEGs and targets. Genes induced by glucose and cAMP in presence of CHX represent only IEGs. Note that CHX is also present in the control condition for this comparison. Target genes were deduced by subtraction between the gene lists.