Histone gene expression and histone mRNA 3' end structure in Caenorhabditis elegans

BMC Molecular Biology

Table 1 Sequence determination of histone mRNA 3' ends.

Histone Type	No. clones	Genomic Sequence	Non-genome encoded tail
H3	1	AACGGCCCTCTTTAGGGCC ACAT	G_[8]AA_[75]
	1	AACGGCCCTCTTTAGGGCC ACAT	G_[2]AA_[162]
	1	AACGGCCCTCTTTAGGGCC ACA	GAA_[102]
	1	AACGGCCCTCTTTAGGGCC ACATAT	AG_[9]AA_[107]
	3	AACGGCCCTCTTTAGGGCC ACA	AA_[67–96]
	1	AACGGCCCTCTTTAGGGCC ACTT	A_[9]GAA_[92]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[31]GAA_[119]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[53]GAA_[84]
H2A	1	AACGGCCCTCTTTAGGGCC ACAT	G_[4]AGAA_[88]
	1	AACGGCCCTCTTTAGGGCC ACAAA	GAA_[96]
	7	AACGGCCCTCTTTAGGGCC ACA	AA_[62–95]
	1	AACGGCCCTCTTTAGGGCC ACAT	AA_[88]
	1	AACGGCCCTCTTTAGGGCC ACATT	AA_[47]
	1	AACGGCCCTCTT-AGGGCC ACA	A_[14]GAA_[84]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[22]GAA_[45]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[83]GAA_[31]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[49]GGAA_[39]
H2B	1	AACGGCCCTCTTTAGGGCC ACAA	GAA_[42]
	2	AACGGCCCTCTTTAGGGCC ACATT	GGAA_[55,63]
	12	AACGGCCCTCTTTAGGGCC ACA	AA_[61–135]
	1	AACGGCCCTCTTTAGGGCC ACAT	AA_[86]
	1	AACGGCCCTCTTTAGGGCC ACT	AA_[77]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[54]GAA_[34]
	1	AACGGCCCTCTTTAGGGCC ACA	A_[51]GAA_[24]
H4	1	AACGGCCCTCTTTAGGGCC ACTT	GAA_[114]
	10	AACGGCCCTCTTTAGGGCC ACA	AA_[37–108]
	3	AACGGCCCTCTTTAGGGCC ACAT	AA_[54–115]
	1	AACGGCCCTCTTTAGGGCC ACATT	AA_[90]
	2	AACGGCCCTCTTTAGGGCC ACTT	AA_[20,74]

Histone mRNA was tailed with ITP and subsequently ATP using poly(A) polymerase, and histone mRNA 3'UTRs were amplified by RT-PCR, cloned and sequenced. Sequences were aligned using the hairpin structure as anchor (in italic). The hairpin structure is followed by genome encoded nucleotides and a non-genome encoded tail added by poly(A) polymerase. The transition point from genomic sequence to non-genomic tail marks the 3' end of histone mRNA that is located three to six nucleotides after the hairpin.

ISSN: 1471-2199