Figure 4From: Three novel ABCC5 splice variants in human retina and their role as regulators of ABCC5 gene expressionSuppression of ABCC5 splice variants by RNA interference increases full length ABCC5 mRNA expression. ARPE-19 cells were transfected with siRNA.1 targeting ABCC5_SV1, ABCC5_SV2 and ABCC5_SV3 or siRNA.2 targeting ABCC5_SV1 and ABCC5_SV2. ARPE-19 transfected with a nonsense siRNA was used as a negative control. Total RNA isolated 8 h, 24 h and 48 h after transfection was used for qRT-PCR analysis. The respective expression values are shown by specific symbols. The expression level of cells transfected with nonsense siRNA was set at 100%. Bar graphs represent standard deviations of reactions performed in triplicate.Back to article page