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Figure 4 | BMC Molecular Biology

Figure 4

From: Three novel ABCC5 splice variants in human retina and their role as regulators of ABCC5 gene expression

Figure 4

Suppression of ABCC5 splice variants by RNA interference increases full length ABCC5 mRNA expression. ARPE-19 cells were transfected with siRNA.1 targeting ABCC5_SV1, ABCC5_SV2 and ABCC5_SV3 or siRNA.2 targeting ABCC5_SV1 and ABCC5_SV2. ARPE-19 transfected with a nonsense siRNA was used as a negative control. Total RNA isolated 8 h, 24 h and 48 h after transfection was used for qRT-PCR analysis. The respective expression values are shown by specific symbols. The expression level of cells transfected with nonsense siRNA was set at 100%. Bar graphs represent standard deviations of reactions performed in triplicate.

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