Effects of leptin and IFNγ on inflammation-related transcripts. RINm5F cells were treated with the indicated cytokines (100 ng/ml leptin; 50 u/ml IL-1β; 25 ng/ml IFNγ) or combinations thereof for 3 h or 16 h before total RNA was isolated. mRNA levels of the indicated genes were assessed by Northern blot hybridisation. GAPDH was used as a control. The RNAs from leptin and/or IL-1 β treated cells were obtained in an experiment independent from that shown in Figure 1. A sample of partially degraded RNA on one of the blots is marked with an x.