Figure 3

Knockdown experiment of hRad9 and p53 using siRNAs. (A) hRad9 and p53 expression after transfection with hRAD9 (siRad9) or TP53 siRNA (siP53). The signals of immunoblots were measured with a densitometer, and the ratios of hRad9 or p53 to actin control were depicted in the figure. (B) p53 expression after transfection with hRAD9 (siRad9) or TP53 siRNA (siP53). (C) Alteration of P21 mRNA expression in the knockdown experiment by hRAD9 or TP53 siRNA. Cells were treated with hRAD9 (siRad9) or TP53 siRNA (siP53) and subjected to UV exposure or mock treatment. The cells were harvested and RNAs were extracted for RT-PCR with P21 primers. The relative amount of P21 mRNA to G3PDH mRNA is shown. Data represent the means ± SD of three independent experiments.