The genes flanking Lsp1 α are dosage compensated, and CG2556 is expressed in the same tissue as Lsp1 α (A) The predicted genes flanking Lsp1 α (exons in black). (B) Northern RNA hybridization analysis of 10 μg of total RNA from embryos 0 – 2 h after laying (E0), embryos 12 h after laying (E12), first instar larvae (L1), second instar larvae (L2), third instar larvae (L3), pupae (P), adult males (M), and adult females (F). All embryo, larval and pupae samples consist of mixed male and female RNA. Northerns were probed with cDNAs for Lsp1 α (a) and rp49 (b). (C) Northern hybridization analysis of 2 μg of poly(A) mRNA from the developmental stages described in (B). Northerns were probed with cDNAs for CG15926 (a), CG2560 (b), CG2556 (c) and rp49 (d). (D) Real-time RT-PCR of Lsp1 α, Lsd-2, Gpdh, rp49, CG2556 and CG2560 in male fat body and whole third instar male larvae cDNA. The fold enrichment of each transcript in fat body compared to whole larvae cDNA is shown. (E)CG2560 and Pgd mRNA was measured by RNase protection relative to rp49 in male and female first instar larvae. Mean female/male transcript ratios and 95% confidence intervals are indicated for 3 experiments. (F) Lsp1 α, CG2556, Pgd and rp49 mRNA was measured by RNase protection in male and female y w staged-third instar larvae. Mean female/male transcript ratios and 95% confidence intervals are indicated for 3 experiments.