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Table 2 Frequencies of G418r colony formation in pDRneo-transfected cells

From: Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics

Cells Frequency of G418r Fold Induction
  + tet - tet  
a 10IN-SCE.1flp1/DRneo, Clone 1 4.7 × 10-6 7.2 × 10-2 1.5 × 104
a 10IN-SCE.1flp1/DRneo, Clone 2 1.3 × 10-6 2.9 × 10-2 2.2 × 104
a 10IN-SCE.1flp1/DRneo, Clone 3 1.0 × 10-6 3.5 × 10-2 3.5 × 104
b Rht14/DRneo 1.3 × 10-5 1.4 × 10-5 1.1
  1. a Three independent pDRneo-transfected 10IN-SCE.1flp1 clones were grown continuously with tet in the medium (+tet) or tet was withdrawn for 48 h. (-tet) prior to G418 selection (methods).
  2. b A single hygror clone, isolated after the transfection of Rht14 cells with pDRneo, was grown for 48 h. and then selected in G418, with tet either present (+tet) or not present (-tet) in the medium throughout (methods).