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Table 2 Frequencies of G418r colony formation in pDRneo-transfected cells

From: Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics

Cells

Frequency of G418r

Fold Induction

 

+ tet

- tet

 

a 10IN-SCE.1flp1/DRneo, Clone 1

4.7 × 10-6

7.2 × 10-2

1.5 × 104

a 10IN-SCE.1flp1/DRneo, Clone 2

1.3 × 10-6

2.9 × 10-2

2.2 × 104

a 10IN-SCE.1flp1/DRneo, Clone 3

1.0 × 10-6

3.5 × 10-2

3.5 × 104

b Rht14/DRneo

1.3 × 10-5

1.4 × 10-5

1.1

  1. a Three independent pDRneo-transfected 10IN-SCE.1flp1 clones were grown continuously with tet in the medium (+tet) or tet was withdrawn for 48 h. (-tet) prior to G418 selection (methods).
  2. b A single hygror clone, isolated after the transfection of Rht14 cells with pDRneo, was grown for 48 h. and then selected in G418, with tet either present (+tet) or not present (-tet) in the medium throughout (methods).
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BMC Molecular Biology

ISSN: 1471-2199

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