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Table 1 Frequencies of Cre-mediated insertion events

From: Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics

a Host cell Insertion construct b Cre Drug Selection c Colonies d GFP-neg e Tet f PCR/SB g I
Rht14-19 pIN-1 + hyg 53 47/48 47/48 10/10 10
Rht14-19 pIN-1 - hyg 0 - - - 0
Rht14-10 pIN-2 + MPA/X 11 9/11 9/11 9/9 1.8
Rht14-10 pIN-2 - MPA/X 0 - - - 0
Rht14-19 pIN-2 + MPA/X 32 22/33 22/33 9/9 4.4
Rht14-19 pIN-2 - MPA/X 0 - - - 0
Rht14-10 pIN2-neoMCS + G418 188 181/188 NT NT 36
Rht14-10 pIN2-neoSCE + G418 169 163/169 3/3 3/3 33
Rht14-10 pIN2-neoR52 + G418 226 217/226 3/3 3/3 43
  1. a Each row represents data for the transfection of 5 × 105 cells.
  2. b Indicates whether pMC-Cre was (+) or was not (-) co-transfected.
  3. c Total number of drug-resistant colonies generated.
  4. d Colonies that did not express GFP.
  5. e GFP-negative colonies that became drug-sensitive on addition of tet.
  6. f GFP-neg/Tets colonies with expected structure detected by PCR or Southern Blot.
  7. g Cre-mediated insertion frequency expressed as events per transfected cell × 105 (assuming all GFP-neg clones are correctly inserted).