A systematic quantification of carbonic anhydrase transcripts in the mouse digestive system

BMC Molecular Biology

Table 1 Primer sequences for quantitative real-time PCR

Gene	Forward primer (5'-3')	Reverse primer (5'-3')	PCR product size (bp)	Tm (°C)
GAPDH	ATGGTGAAGGTCGGTGTG	CATTCTCGGCCTTGACTG	186	56
Car1	TTGATGACAGTAGCAACC	CCAGTGAACTAAGTGAAG	161	51
Car2	CAAGCACAACGGACCAGA	ATGAGCAGAGGCTGTAGG	122	56
Car3	GCTCTGCTAAGACCATCC	ATTGGCGAAGTCGGTAGG	160	54
Car4	CTCCTTCTTGCTCTGCTG	GACTGCTGATTCTCCTTA	145	55
Car5a	ACCAAAGCAAGGGCATACAG	TGGCACAGAGAAGTCCCACA	104	58
Car5b	AATGGCTTGGCTGTGATAGG	GGCGTAGTGAGAGACCCAGA	187	60
Car6	AAGATTGACGAGTATGCC	TAGGTGTAATAGTGGTGG	145	54
Car7	CAATGACAGTGATGACAGAA	TCCAGTGAACCAGATGTAG	160	55
Car9	CTGAAGACAGGATGGAGAAG	GCAGAGTGCGGCAGAATG	221	57
Car12	CCTATGTTGGTCCTGCTG	CGTTGTAACCTTGGAACTG	143	53
Car13	AATACGACTCCTCACTCC	TGCCGCAACCTGTAGTTC	116	52
Car14	TGTTGTTCTTCGCTCTCCTG	CACTGTCTGTCTGGATATTG	161	53
Car15	AGCACAGCCTGGATGAGA	CAGACACAATGGCAGAGA	170	55

ISSN: 1471-2199