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Table 2 Primer sequences and PCR conditions for amplifying coding sequence of OPTC

From: Evaluation of the OPTC gene in primary open angle glaucoma: functional significance of a silent change

Primer Sequence Location Anneal temp. (°C) Mg2+conc. (mM) Length of PCR product (bp)
Forward (5'-3') Reverse (5'-3')     
2aF-GTGGAGTAAATCTGCGAGCC 2aR-GGCAGAACTTCAAAGGAATC Exon 2 50 1.5 264
2bF-CTGAAGGGATTCTCAGTCCCATCTG 2bR-CTCCCAGTGTCATGCAGGGAATGT Exon 2 62 2.0 313
3F-GTTCCCAGAGTCCAAAGTTAAG 3R-GGTCATAGCTGCTTGGCCTATG Exon 3 54 1.5 275
4F-CAGATGGCCATGGTTCAGACAC 4R-GTGTTGGTGACTGTCCTAGTG Exon 4 58 2.0 333
5F-GACAAGGAAAGATAGTGTGTTCTG 5R-GTGGGTGGTGGAGGTGATAGATAG Exon 5 60 2.0 310
6F-CCACCTGTCTCTGGTCTCATAG 6R-CATCCTCCACGCTGTTTATCACC Exon 6 56 1.5 233
7F-CTGATGTGAGCCTTTGGTGCTGC 7R-GATAAGGAATTGGGCTTTATGGC Exon 7 62 2.0 334