Table 2 Primer sequences and PCR conditions for amplifying coding sequence of OPTC
Primer Sequence | Location | Anneal temp. (°C) | Mg2+conc. (mM) | Length of PCR product (bp) | |
|---|---|---|---|---|---|
Forward (5'-3') | Reverse (5'-3') | Â | Â | Â | Â |
2aF-GTGGAGTAAATCTGCGAGCC | 2aR-GGCAGAACTTCAAAGGAATC | Exon 2 | 50 | 1.5 | 264 |
2bF-CTGAAGGGATTCTCAGTCCCATCTG | 2bR-CTCCCAGTGTCATGCAGGGAATGT | Exon 2 | 62 | 2.0 | 313 |
3F-GTTCCCAGAGTCCAAAGTTAAG | 3R-GGTCATAGCTGCTTGGCCTATG | Exon 3 | 54 | 1.5 | 275 |
4F-CAGATGGCCATGGTTCAGACAC | 4R-GTGTTGGTGACTGTCCTAGTG | Exon 4 | 58 | 2.0 | 333 |
5F-GACAAGGAAAGATAGTGTGTTCTG | 5R-GTGGGTGGTGGAGGTGATAGATAG | Exon 5 | 60 | 2.0 | 310 |
6F-CCACCTGTCTCTGGTCTCATAG | 6R-CATCCTCCACGCTGTTTATCACC | Exon 6 | 56 | 1.5 | 233 |
7F-CTGATGTGAGCCTTTGGTGCTGC | 7R-GATAAGGAATTGGGCTTTATGGC | Exon 7 | 62 | 2.0 | 334 |