Figure 3
From: NF-κB mediates the transcription of mouse calsarcin-1 gene, but not calsarcin-2, in C2C12 cells
Reporter analysis of the mouse CS1 and CS2 gene promoters. A. Reporter analysis of mouse CS1 gene promoter deletion constructs. B. Reporter analysis of mouse CS2 gene promoter deletion constructs. The 5'-deleted promoter segments were generated as described in Materials and Methods. C2C12 cells were co-transfected with various promoter regions fused to firefly luciferase and with a Renilla luciferase expression control vector. The resulting firefly luciferase activity was then normalized to Renilla luciferase activity and the relative values are presented as the fold-increase over the activity of the promoterless pGL3-basic vector. The length of each 5'-deletion fragment is calculated relative to the translation initiation ATG codon and is indicated to the left of each bar. The presence of putative NF-κB binding elements is also indicated. Values represent the mean ± SD of three independent experiments.