Figure 3

Determination of the initiation of transcription site. Three primers behind the cryptic promoter were designed to run the RT-PCR. Primer 1 was used in Lanes 1, 4, and 7; Primer 2 in Lanes 2, 5, and 8; Primer 3 in Lanes 3, 6, and 9. M stands for the molecular marker. The "+" stands for the positive PCR control. The "-" marker stands for the negative PCR control. Lane 1, Lane 2, and Lane 3 contain the RT-PCR product from pgR106gp53 transformed E. coli. Lane 4, Lane 5, and Lane 6 for the RT-PCR product with template RNA from the gpR106VP1 transformed E. coli. Lane 7, Lane 8, and Lane 9 for the PCR product with total RNA from the pgR106gp53 transformed E. coli. No PCR product was amplified, showing the RNA did not contain plasmid DNA.