From: Selection of reliable reference genes for qPCR studies on chondroprotective action
Experimental condition | mRNA relative to untreated control group (mean ± SD) | |||||
---|---|---|---|---|---|---|
 | ACTB | GAPDH | B2M | HPRT1 | SDHA | YWHAZ |
IL-1β | 1.5 ± 0.63 | 1.2 ± 0.57 | 1.10 ± 0.08 | 1.23 ± 0.46 | 1.02 ± 0.66 | 0.89 ± 0.45 |
Simultaneous incubation with diacerein [c]1 | 1.03 ± 0.39 | 1.11 ± 0.40 | 1.36 ± 0.29 | 1.26 ± 0.40 | 1.13 ± 0.34 | 1.14 ± 0.43 |
Simultaneous incubation with diacerein [c]2 | 0.71 ± 0.18 | 1.02 ± 0.21 | 1.15 ± 0.20 | 1.07 ± 0.25 | 0.88 ± 0.17 | 0.69 ± 0.17 |
Pre-incubation with diacerein [c]1 | 1.21 ± 0.21 | 1.12 ± 0.33 | 1.23 ± 0.57 | 1.29 ± 0.40 | 1.35 ± 0.39 | 1.11 ± 0.31 |
Pre-incubation with diacerein [c]2 | 0.56 ± 0.07 | 0.79 ± 0.11 | 0.96 ± 0.12 | 1.00 ± 0.13 | 0.98 ± 0.19 | 0.7 ± 0.15 |
Diacerein only | 0.43 ± 0.06* | 0.63 ± 0.10 | 0.73 ± 0.09 | 0.87 ± 0.15 | 0.88 ± 0.15 | 0.59 ± 0.07 |