Skip to main content

Table 5 Relative mRNA levels of C-28/I2 chondrocytes under the influence of IL-1β and diacerein.

From: Selection of reliable reference genes for qPCR studies on chondroprotective action

Experimental condition

mRNA relative to untreated control group (mean ± SD)

 

ACTB

GAPDH

B2M

HPRT1

SDHA

YWHAZ

IL-1β

1.5 ± 0.63

1.2 ± 0.57

1.10 ± 0.08

1.23 ± 0.46

1.02 ± 0.66

0.89 ± 0.45

Simultaneous incubation with diacerein [c]1

1.03 ± 0.39

1.11 ± 0.40

1.36 ± 0.29

1.26 ± 0.40

1.13 ± 0.34

1.14 ± 0.43

Simultaneous incubation with diacerein [c]2

0.71 ± 0.18

1.02 ± 0.21

1.15 ± 0.20

1.07 ± 0.25

0.88 ± 0.17

0.69 ± 0.17

Pre-incubation with diacerein [c]1

1.21 ± 0.21

1.12 ± 0.33

1.23 ± 0.57

1.29 ± 0.40

1.35 ± 0.39

1.11 ± 0.31

Pre-incubation with diacerein [c]2

0.56 ± 0.07

0.79 ± 0.11

0.96 ± 0.12

1.00 ± 0.13

0.98 ± 0.19

0.7 ± 0.15

Diacerein only

0.43 ± 0.06*

0.63 ± 0.10

0.73 ± 0.09

0.87 ± 0.15

0.88 ± 0.15

0.59 ± 0.07

  1. Boldfaced values p < 0.05
  2. * p < 0.05 and change > 2-fold
  3. mRNA levels under the treatment with diacerein in different experimental conditions are presented relative to the untreated control group calculated by the equation E-ΔCt (Mean ± SD, n = 9; value of 1 represents average level of mRNA expression in untreated control group). [c]1, low concentration of diacerein (5 μM); [c]2, high concentration of diacerein (50 μM).