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Table 3 Relative mRNA levels of C-28/I2 chondrocytes under the influence of IL-1β and glucosamine.

From: Selection of reliable reference genes for qPCR studies on chondroprotective action

Experimental condition

mRNA relative to untreated control group (mean ± SD)

 

ACTB

GAPDH

B2M

HPRT1

SDHA

YWHAZ

IL-1β

1.30 ± 0.30

1.05 ± 0.27

1.14 ± 0.22

1.08 ± 0.24

1.00 ± 0.20

0.88 ± 0.16

Simultaneous incubation with glucosamine [c]1

1.27 ± 0.24

1.13 ± 0.18

1.35 ± 0.22

0.93 ± 0.13

0.83 ± 0.14

0.83 ± 0.15

Simultaneous incubation with glucosamine [c]2

0.63 ± 0.16

0.69 ± 0.09

1.04 ± 0.11

0.56 ± 0.06

0.77 ± 0.15

0.61 ± 0.11

Pre-incubation with glucosamine [c]1

0.87 ± 0.11

0.97 ± 0.25

1.11 ± 0.22

0.66 ± 0.16

1.02 ± 0.11

0.74 ± 0.10

Pre-incubation with glucosamine [c]2

0.28 ± 0.05*

0.43 ± 0.07*

0.67 ± 0.15

0.37 ± 0.08*

0.35 ± 0.06*

0.37 ± 0.11*

Glucosamine only

0.60 ± 0.27

0.66 ± 0.22

0.77 ± 0.34

0.15 ± 0.05*

0.20 ± 0.07*

0.37 ± 0.28*

  1. Boldfaced values p < 0.05
  2. *p < 0.05 and change > 2-fold
  3. mRNA levels under the treatment with glucosamine in different experimental conditions are presented relative to the untreated control group calculated by the equation E-ΔCt (Mean ± SD, n = 9; value of 1 represents average level of mRNA expression in untreated control group). [c]1, low concentration of glucosamine (50 μM); [c]2, high concentration of glucosamine (5 mM).
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BMC Molecular Biology

ISSN: 1471-2199

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