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Table 5 Comparison of the four protocols for quantity and quality of extracted RNA for only samples that produced biologically-useful RNA (RIN ≥ 1.4) in ten year old FFPE samples. Case success is defined for each protocol as the percentage of samples where at least one of three RNA extraction attempts provided a RIN ≥ 1.4.

From: RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Ten year old samples:   Protocol 1 Protocol 2 Protocol 3 Protocol 4
FFPE tissue input   80 μm 10 μm 20 μm 50 μm
Successful extractions   26/42 (62%) 26/42 (62%) 35/42 (83%) 30/42 (71%)
Case success   12/14 (86%) 14/14 (100%) 13/14 (93%) 13/14 (93%)
RNA (pg/μl) Minimum value 1,236 1,650 1,314 9,864
  Maximum value 73,861 165,588 137,594 314,693
  Mean 17,754 51,985 30,160 107,042
  Median 13,692 31,104 20,229 86,682
Minimum RIN of 1.4 Minimum value 1.4 1.5 1.9 1.8
  Maximum value 2.5 5.2 4.4 6.1
  Mean 2.0 2.5 2.3 2.4
  Median 2.1 2.3 2.3 2.2