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Figure 5 | BMC Molecular Biology

Figure 5

From: Regulation of heme oxygenase-1 mRNA deadenylation and turnover in NIH3T3 cells by nitrosative or alkylation stress

Figure 5

Effect of MMS on HO-1 mRNA deadenylation and stability. A. NIH3T3 cells were treated as controls or with 1 mM MMS for 6 h, and total RNA samples (20 μg and 7.5 μg, respectively) were assayed for HO-1 mRNA deadenylation. Residual full-length mRNA and the 3' end fragment carrying the poly(A) tail are indicated. FD represents a fully deadenylated 3' fragment of HO-1 mRNA. B. The size of the poly(A) tail was estimated as for Fig. 5. C. A fraction of these samples was used without further modification to assess the half-life of HO-1 mRNA. Quantification in panels B and C shows the fitted decay lines calculated from two independent experiments. Data points show the mean and standard errors.

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