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Table 1 Oligonucleotides used to generate constructs of the 3'-UTR of the human CDK5R1 gene.

From: The 3' untranslated region of human Cyclin-Dependent Kinase 5 Regulatory subunit 1 contains regulatory elements affecting transcript stability

Fragment

nt (bp)

Sequence

PCR annealing (°C)

UTR

1–2687

Fw: 5' GC TCTAGA ATCGGTGAGCACTGTGCCTG 3'

Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3'

62

C1

1–661

Fw: 5' GCTCTAGA ATCGGTGAGCACTGTGCCTG 3'

Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3'

62

C2

562–1226

Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3'

Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3'

58

C2.1

562–661

Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3'

Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3'

58

C2.2

642–881

Fw: 5' GCTCTAGA CTACCCCCTTGTCTGCTGCT 3'

Rev: 5' GCTCTAGA GTATGGCATCCCTCACCTTG 3'

58

C2.3

862–1226

Fw: 5' GCTCTAGA CAAGGTGAGGGATGCCATAC 3'

Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3'

56

C3

1167–1408

Fw: 5' GCTCTAGA GTTTCCACCTTACCCTACTG 3'

Rev: 5' GCTCTAGA AGTGACCCTCTGCCTCCCC 3'

58

C4

1368–1688

Fw: 5' GCTCTAGA TGCTGGAATAGGGACCTGG 3'

Rev: 5' GCTCTAGA GTGCTGTGTGAAGTCTGTG 3'

56

C5

1619–2326

Fw: 5' GCTCTAGA GGGACTGTCAGATAATCGGTG 3'

Rev: 5' GCTCTAGA TCCAGGTTTACAAGAAAAAGAGAA 3'

62

C6

2273–2687

Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3'

Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3'

56

C6del

2273–2636

Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3'

Rev: 5' GCTCTAGA CAGCCAAACTGAGCTTCATG 3'

58

  1. XbaI recognition site sequence is underlined