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Table 1 Oligonucleotides used to generate constructs of the 3'-UTR of the human CDK5R1 gene.

From: The 3' untranslated region of human Cyclin-Dependent Kinase 5 Regulatory subunit 1 contains regulatory elements affecting transcript stability

Fragment nt (bp) Sequence PCR annealing (°C)
UTR 1–2687 Fw: 5' GC TCTAGA ATCGGTGAGCACTGTGCCTG 3' Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3' 62
C1 1–661 Fw: 5' GCTCTAGA ATCGGTGAGCACTGTGCCTG 3' Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3' 62
C2 562–1226 Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3' Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3' 58
C2.1 562–661 Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3' Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3' 58
C2.2 642–881 Fw: 5' GCTCTAGA CTACCCCCTTGTCTGCTGCT 3' Rev: 5' GCTCTAGA GTATGGCATCCCTCACCTTG 3' 58
C2.3 862–1226 Fw: 5' GCTCTAGA CAAGGTGAGGGATGCCATAC 3' Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3' 56
C3 1167–1408 Fw: 5' GCTCTAGA GTTTCCACCTTACCCTACTG 3' Rev: 5' GCTCTAGA AGTGACCCTCTGCCTCCCC 3' 58
C4 1368–1688 Fw: 5' GCTCTAGA TGCTGGAATAGGGACCTGG 3' Rev: 5' GCTCTAGA GTGCTGTGTGAAGTCTGTG 3' 56
C5 1619–2326 Fw: 5' GCTCTAGA GGGACTGTCAGATAATCGGTG 3' Rev: 5' GCTCTAGA TCCAGGTTTACAAGAAAAAGAGAA 3' 62
C6 2273–2687 Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3' Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3' 56
C6del 2273–2636 Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3' Rev: 5' GCTCTAGA CAGCCAAACTGAGCTTCATG 3' 58
  1. XbaI recognition site sequence is underlined