Fragment | nt (bp) | Sequence | PCR annealing (°C) |
---|---|---|---|
UTR | 1–2687 | Fw: 5' GC TCTAGA ATCGGTGAGCACTGTGCCTG 3' Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3' | 62 |
C1 | 1–661 | Fw: 5' GCTCTAGA ATCGGTGAGCACTGTGCCTG 3' Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3' | 62 |
C2 | 562–1226 | Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3' Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3' | 58 |
C2.1 | 562–661 | Fw: 5' GCTCTAGA TGAGCGGGTCTAGTGGAAAG 3' Rev: 5' GCTCTAGA AGCAGCAGACAAGGGGGTAG 3' | 58 |
C2.2 | 642–881 | Fw: 5' GCTCTAGA CTACCCCCTTGTCTGCTGCT 3' Rev: 5' GCTCTAGA GTATGGCATCCCTCACCTTG 3' | 58 |
C2.3 | 862–1226 | Fw: 5' GCTCTAGA CAAGGTGAGGGATGCCATAC 3' Rev: 5' GCTCTAGA GAAAGAAAATCAATAAAGTACAC 3' | 56 |
C3 | 1167–1408 | Fw: 5' GCTCTAGA GTTTCCACCTTACCCTACTG 3' Rev: 5' GCTCTAGA AGTGACCCTCTGCCTCCCC 3' | 58 |
C4 | 1368–1688 | Fw: 5' GCTCTAGA TGCTGGAATAGGGACCTGG 3' Rev: 5' GCTCTAGA GTGCTGTGTGAAGTCTGTG 3' | 56 |
C5 | 1619–2326 | Fw: 5' GCTCTAGA GGGACTGTCAGATAATCGGTG 3' Rev: 5' GCTCTAGA TCCAGGTTTACAAGAAAAAGAGAA 3' | 62 |
C6 | 2273–2687 | Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3' Rev: 5' GCTCTAGA GAATCAGCACAGTACAAAAATAAAT 3' | 56 |
C6del | 2273–2636 | Fw: 5' GCTCTAGA CCACAGGAATAATAGTTCAGG 3' Rev: 5' GCTCTAGA CAGCCAAACTGAGCTTCATG 3' | 58 |