Skip to main content
Figure 3 | BMC Molecular Biology

Figure 3

From: Tissue factor pathway inhibitor-2 was repressed by CpG hypermethylation through inhibition of KLF6 binding in highly invasive breast cancer cells

Figure 3

KLF6 can transactivate and binds to the promoter of TFPI-2. (A) The p-144 promoter-luciferase activity induced by cotransfection with various amount of KLF6 expression plasmids in MDA-MB-435 cells. The KLF6 cDNA was a generous gift of Scott Friedman and was cloned into PCDNA 3.0 expression vector. (B) Both the predicted KLF6 binding sequence (K6) and the authentic KLF6 binding (Kc) [46] can form specific bands with nuclear extract (NE) from MCF-7 cell lines. And the specific bands were supershifted by the antibody of KLF6. (C) No difference of K6 binding activity was found between MCF-7 and MDA-MB-435 cell lines. Lane 1 to 6: NE from MCF-7; lane 7, 8: NE from MDA-MB-435. Lane 5, 7: 2.5 μg NE; lane 6, 8: 5 μg NE.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us