Figure 5

Electrophoretic mobility shift assays (EMSA) with selected upstream DNA fragments of PTS coding regions using the purified SugR protein. The physical maps of the extended fructose-PTS gene cluster as well as of the genes ptsG and ptsS are shown. Beneath the maps the fluorescently labelled PCR fragments are indicated which were used for EMSA studies. These studies were carried out with 15 pmol of purified SugR protein and 0.05 pmol of labeled PCR fragments. The results obtained for each PCR fragment are presented by agarose gel photos. In each picture, the left lane shows the shift in presence of the SugR protein, whereas the right lane shows the negative control without added SugR protein. Transcriptional terminators are denoted as stem loop structures.