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Table 1 Oligonucleotides used in PCR-based site-directed mutagenesis

From: Cooperation between MEF2 and PPARγ in human intestinal β,β-carotene 15,15'-monooxygenase gene expression

Primer sequence

Mutated sitea

5'-GGAAATTAA CGC TTAACCA AAC-3'

PPAR/RXR -52/-50

5'-TAGTCTGAA AGT AGC TTTT-3'

C/EBP -161/-159

5'-TCTGCTT C TTTA C AACCTAGTCTG-3'

MEF2 -179/-174

5'-ACTCCTG TG CTCAAGTG TG CC G-3'

CRE/RAR -633/-623

  1. aInitiation site for BCMO1 transcription, +1. The mutated nucleotides are in bold.
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BMC Molecular Biology

ISSN: 1471-2199

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