Characterizing exons 11 and 1 promoters of the mu opioid receptor (Oprm) gene in transgenic mice

BMC Molecular Biology

Table 1 Distribution of E11 and E1 promoter activities in the transgenic CNS

Structure	tau/LacZ (E11 promoter)	E11-LI	tau/GFP (E1 promoter)	E4-LI
Cerebral cortex
Piriform cortex	+++	-	+++	++
Lateral parietal association cortex	+	-	++	+
Primary motor cortex	+	-	+	+
Olfactory system
Olfactory bulb	++	-	+	+
Olfactory tubercule	+	++	+/-	+
Striatum	+	+	+	++
Nucleus accumbens	+	+	+	++
Globus pallidus	+	+	+	+
Ammon's horn
CA1	++	-	+/-	+
CA2	++	-	+/-	+
CA3	+++	-	+++	++
Pyramidal cell layer	+	-	+	+
Polymorph layer dentate gyrus	++	-	+	+
Subthalamic nucleus	+/-	-	+++	++
Zona incerta	+	-	+	-
Thalamus
Laterodorsal thalamic nucleus	++	-	+/-	+
Lateral posterio thalamic nucleus	++	-	+/-	+
Ventral posteromedial thalamic nucleus	+	-	++	+/-
Ventral posterolateral thalamic nucleus	+	-	++	+/-
Hypothalamus	+	-	+	+
Substantia nigra, reticular	++	++	+/-	+/-
Substantia nigra, compact	+++	-	++	+
Locus coeruleus	+	-	+	++
Cerebellum
Purkinje cell	+++	-	+++	+++
Medial cerebellar nucleus	++	-	++	++
Brainstem	++	-	++	++
Spinal cord
Laminae I-II	+	-	+++	+++
Laminae III-IV	+	-	++	+
Laminae V-VI	+	-	++	+
Laminae VII-VIII	+	-	++	+
Laminae IX	+	-	++	+

E11 promoter activity indicated by X-gal staining of tau/LacZ fusion protein, E1 promoter activity indicated by GFP imaging of tau/GFP fusion protein and exon 4-like immunoreactivity (E4-LI) shown by immunostaining with an E4 antibody in the selected areas of the transgenic mice are presented. The intensity of X-gal staining, green fluorescent protein signal and Alex568 fluorescence of MOR-1-LI was visually estimated as high (+++), moderate (++), low (+), very low (+/-), and negative (-). Exon 11-like immunoreactivity (E11-LI) is from the literature[58].

ISSN: 1471-2199