Saccharomyces cerevisiae Esc4 has six BRCT motifs that are conserved in both sequence and domain architecture among yeast homologs. The six BRCT motifs in Esc4 are indicated with brackets and yellow color and were described previously . The motifs in Esc4 are shown as they align with a subset of Esc4 homologs: three from other budding yeasts Ashbya gossipii, Kluyveromyces lactis and Candida glabrata and one from the evolutionarily distant fission yeast Schizosaccharomyces pombe Brc1. The highly conserved aromatic residue, a hallmark of BRCT motifs, is marked above the alignment with a red asterisk. Blue color indicates similarity with the predominant residue at the position in the alignment, with darkness correlated with greater similarity as calculated by ClustalW software and displayed with Jalview alignment editor's BLOSUM62 score viewing option [67, 68].