Figure 8

LAT promoter activity in various cell-types. A. Reporter constructs containing the indicated regions of the human LAT promoter were transiently transfected into the indicated cell-types. Following incubation, samples were harvested and after normalization to Renilla luciferase activity fold activation was calculated relative to that obtained with the promoterless pGL3-basic vector which was set at 1-fold. B. RBL-2H3 cells were transiently transfected with either the wild-type -1000 to -1 reporter plasmid or reporter plasmids containing the -1000 to -1 fragment with the indicated site(s) mutated. Following incubation, samples were harvested and after normalization to Renilla luciferase activity, the activity obtained with the wild-type promoter was set at 100%. p < 0.05 for mutant reporters relative to wild-type using the two-tailed t test C. RBL-2H3 cells were transfected with the -1000 to -1 or -1000 to -200 reporter plasmids. Following incubation, samples were harversted, luciferase activities were measured and normalized to Renilla luciferase, and the activity obtained with empty pGL3-basic was set at 1-fold. For Fig. 8A-8C, the results shown are the average fold induction and s.e.m. from three or more independent experiments. For 8B and 8C, p < 0.05 for the mutant reporters relative to wild-type as determined using the two-tailed t test.