Figure 2

Mapping the 5' and 3' boundaries of the LAT promoter. A.-D. Jurkat T cells were transiently transfected with a pGL3-basic firefly luciferase reporter vector containing various DNA fragments extending upstream of the translation start site for the human LAT gene. Cells were also co-transfected with the internal control pRL-TK Renilla luciferase plasmid. Following incubation, cells were harvested, luciferase activity was quantitiated and after normalization of each sample based on Renilla luciferase activity, the fold activation relative to the empty pGL3-basic vector was calculated. Results presented are the average fold induction and the standard error of the mean (s.e.m.) from three or more independent experiments. For each graph, the human LAT promoter fragments inserted into pGL3-basic are shown schematically on the left. Arrows depict the two clusters of transcription start sites for the LAT gene.