Figure 2From: Specific use of start codons and cellular localization of splice variants of human phosphodiesterase 9A geneDifferential start codon usage in PDE9A splice variants. (A) Western blot analysis of the expression of the chimeric PDE9A splice variants fused to GFP. GFP-fused proteins of PDE9A1 (lane 1, 96 kDa), the N-terminal region of PDE9A1 (lane 2, 62 kDa), PDE9A2 (lane 3, 89 kDa), PDE9A3 (lane 4, 81 kDa), and PDE9A17 (lane 5, 91 kDa) were produced in transfected Cos-1 cells, and were immunoblotted using a rabbit anti-GFP serum (1:1000). (B) Sequence comparison between Kozak consensus motif and PDE9A start codons, where the critical -3 and +4 positions are marked. (C) Schematic secondary structure representation of PDE9A exon 1 sequence. Start codons are emphasized by grey boxes.Back to article page