Gene structure and expression of At MND1. (A) Line diagram showing the site of T-DNA insertion in the AtMND1 gene. Exons and the UTR's are indicated as black boxes and clear boxes respectively. Inverted triangle in the seventh intron represents the site of T-DNA insertion. The black and white arrows represent the LB1 and the gene-specific primers used to genotype plants. The black bar below the gene represents the Mnd1 domain in the protein. (B) Real Time RT-PCR analysis of relative expression of AtMND 1 in somatic and reproductive tissues. The constitutive GAPC gene was used as the normalization control.