Figure 1

Sequence alignment of the human acyl-CoA synthetases long-chain family members. The succession of clear-shaded blocks overlaid on the alignment represents the exon organization of the respective mRNA. Conserved residues are in bold. Position of the P-loop, Gate, A-box and Linker motifs is indicated. Y and F residues of the two alternative Gate domains are boxed and indicated with a star. Transmembrane spanning segments predicted with the highest score by TopoPrediction, SOSUI and TMHMM are underlined. For clarity, ACSL3 variant 1 and 2, and ACSL5 variant 2 and 3, which encode the same isoform, are annotated as ACSL3_v1v2 and ACSL5_v2v3, respectively. Alignment was performed with MUTALIN [14]. Protein length (residues): ACSL1_v1, 698; ACSL1_v2, 698; ACS3_v1v2, 720; ACSL4_v1, 670; ACSL4_v2, 711; ACSL5_v1, 739; ACSL5_v2v3, 683; ACSL6_v1, 722; ACSL6_v2, 722; ACSL6_v3, 622. GenBank accessions numbers are given in Table 2.