Western blot analysis of B. subtilis cells using anti-GFP antiserum. Lane 1: PY79, exponential growth, lanes 2 and 3: JN4 (sbcD-gfp), lane 2 exponential growth, lane 3 one hour after addition of MMC (100 ng/ml), lanes 4 and 5: JN7 (addA-gfp), lane 4 exponential growth, lane 5 one hour after addition of MMC, lanes 6 and 7: JM41 (addB-yfp), lane 6 exponential growth, lane 7 one hour after addition of MMC, lane 8: PY79, lane 9: DK39 (gfp-recA), lanes 10 and 11: JN6 (sbcC-yfp), lane 10 exponential growth, lane 11 one hour after addition of MMC. The same amount of protein was loaded in each lane. Arrows indicate the position of fusion proteins and of free GFP. AddA-GFP: 169 kDA, AddB-YFP: 162 kDa, SbcC-YFP: 153 kDa, SbcD-GFP: 71 kDa, GFP-RecA: 66 kDa, GFP: 28 kDa. Note that the antiserum strongly cross reacts with a protein of about 48 kDa.