Figure 9
Effect of in vitro DNA methylation on the podocalyxin promoter-driven luciferase expression. Transient transfections were performed in HEK293 cells. Results represent mean ± SEM from three independent experiments performed in duplicates. Promoter activity of each transfection was expressed as a ratio of luciferase/renilla activities. Data are expressed as arbitrary units calculated as fold increases over void pGL-3 enhancer vector activity, which is assigned a value of 1.0. *p < 0.05 vs. mock methylated control.