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Figure 2 | BMC Molecular Biology

Figure 2

From: Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoter

Figure 2

Western blots of human podocalyxin and Sp1 transcription factor. Cell lysates (20 μg) were resolved on SDS-7.5% polyacrylamide gels under reducing conditions, transferred to PVDF membranes and immunoblotted with either 1 μg/ml of anti-podxl or anti Sp1 antibodies. β-Actin content was blotted as a control of gel loading. The experiment was repeated three times.

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