Figure 2From: Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoterWestern blots of human podocalyxin and Sp1 transcription factor. Cell lysates (20 μg) were resolved on SDS-7.5% polyacrylamide gels under reducing conditions, transferred to PVDF membranes and immunoblotted with either 1 μg/ml of anti-podxl or anti Sp1 antibodies. β-Actin content was blotted as a control of gel loading. The experiment was repeated three times.Back to article page