Figure 2
From: Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR
Primer-template dependent inhibitory effect on mRNA amplification. RNA samples extracted from cells harvested at 2,1 mg (dry weight)/mL were treated with DNase I according to protocol II. Different set of primers (as described in table I) was used to amplify CNA2 (lane 2), ACT1 (lane 3), TPS2 (lane 4), PDA1 (lane 5), CNA1 (lane 6), TPS1 (lane 7) mRNAs. 100 bp DNA ladder (lane P) and RT-minus control using ACT1 primers (lane 1).