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Archived Comments for: SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis

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  1. Methodology - explanation reqd...

    Natarajan Ganesan, Georgetown University

    21 April 2006

    How is the match found for a given sequence? Do you connect the program to a database of restriction enzymes? I couldnt find the description in Methods.

    Whatever be the approach to find a match, I feel the need to connect a restriction enzyme database to compute the ideal enzyme and an ideal insert.

    As I see, one possible approache would allow the user to select an enzyme and then look for a match that retains the amino-acid sequence (using degeneracy).

    Sincerely Yours

    Natarajan Ganesan, PhD


    Competing interests

    No competing interests