Table 3 Evaluation of the usefulness of six potential reference genes in eight tissues of Atlantic salmon ranked by the geNorm software. 1 = best, 6 = worst. Six individuals were analyzed for six genes in eight tissues.
From: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Tissue | Muscle | Liver | Gills | Head kidney | Spleen | Brain | Intestine | Thymus | Total ranking |
|---|---|---|---|---|---|---|---|---|---|
18S rRNA | 3 | 5 | 6 | 2 | 3 | 5 | 6 | 4 | 4 |
S20 | 6 | 4 | 5 | 5 | 2 | 3 | 5 | 5 | 5 |
β-actin | 2 | 3 | 4 | 4 | 4 | 4 | 1 | 2 | 3 |
GAPDH | 5 | 6 | 3 | 6 | 6 | 6 | 3 | 6 | 6 |
EF1AA | 4 | 2 | 2 | 3 | 5 | 1 | 3 | 3 | 2 |
EF1AB | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 |