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Figure 2 | BMC Molecular Biology

Figure 2

From: Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

Figure 2

Effects of zinc chelation on KLF15 Binding. A. EMSA using 32P-labeled oligomers (bRho29) containing -96 to -66 bp fragment from bovine rhodopsin promoter. Lane 1, GST protein; Lane 2, KLF15-ZF-GST protein, Lanes 3 -7 same as 2 with EDTA and ZnCl2 (mM) as indicated. B. Same as A, but with MgCl2. C. Same as A, but with EGTA and ZnCl2. The altered migration of the unbound oligonucleotides is attributable to the salt effects of high concentrations of EGTA.

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BMC Molecular Biology

ISSN: 1471-2199

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