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Table 3 Structural motifs used for the Bacillus anthracis tmRNA mode

From: Comparative 3-D Modeling of tmRNA

Motif

SCOR class

tmRNA Res.

Source Res.

Coordinates

Comments

1

 

1–11, 12–27, 316–355

1–11, 13–28, 324–363

E. coli 1

ACCA end and G3-U349 pair

2

loop with stacked interdigitated bases

28–33, 312–316

477–482, 451–455

1J5E.pdb

 

3

 

300–301

 

ERNA-3D

 

4a

 

43–53, 61–65, 294–299

43–53, 63–67, 302–308

E. coli model

helix 2d, helix 3

4b

pseudoknot

58–60, 66, 67–73

12–14, 20, 23–29,

1RNK.pdb

pk1, helix 4b

4c

 

54–56, 74–75

54–56, 77–78

E. coli 6a

pk1, helix 4a

5

heptaloop

82–88

335–341

1JJ2.pdb

 

6a

one looped out A

A112

210

1GID.pdb

 

6b

 

C114, C127

 

ERNA-3D

 

6c

octaloop

117–124

1499–1506

1JJ2.pdb

 

7a

 

134–138, U163, A164, 165–169,

140–144, A171, A174, 175–179

E. coli 10b

helix 6a, looped out U163 and A164.

7b

one looped out U

U158

U87

1JJ2.pdb

 

7c

 

144–147, 148–156, 180–185

259–262, 264–272, 293–298

E. coli 13b

helix 6c, helix 7, pk2

8

 

G133, C170, 171–177, 178–179

C138, G181, 182–188, 190–191

E. coli 10b

 

9a

 

C190, 191–198, 208–215, 216

G200, 203–210, 219–226, U229

E. coli 11a, 11b

purine-rich loop, pk3

9b

pseudoknot

202–206, 228–232

9–13, 28–32

1RNK.pdb

pk3, helix 9

10a

 

199–201

154–156

E. coli 6a

pk3

10b

 

217–227

229–239

E. coli 6a

pk3

11a

one looped out C

C269

C6

1BVJ.pdb

 

11b

stacked duplexes with four non-WC pairs

241–245, 260–263

795–798, 815–818

1JJ2.pdb

 

12

 

246–259, 275–290

259–277, 284–299

E. coli 6a

helix 10c, helix 11ab, pk4

  1. Motif numbers in bold, their SCOR classification [12], residue positions in the tmRNA model and the source structure are shown in columns one to four. Column five lists the filenames containing the atomic coordinates that were derived from the PDB [31], the E. coli model (see Table 2), or were generated by ERNA-3D [11].