Table 1 S. pombe strains used

P138

ade6-M210 leu1-32 ura4-D18 h ^-

this work

P903

spp1-GFP::kan ^r ade6-M120 leu1-32 ura4-D18 h ^-

[25]

P1083

cdc45(sna41)-YFP::ura4 ⁺ ade6-M210 leu1-32 ura4-D18 h ^-

[25]

P1100

cdc45(sna41)-YFP::ura4 ⁺ nmt1*cdc23-1E2-td::kan ^r h ^-

this work

P1171

mcm7-GFP _{N 760} ::Tcs ::ura4 ⁺ ade6-M210 leu1-32 ura4-D18 h ^- [pREP3X-TEV-NLS(LEU2)]

this work

P1205

spp1-GFP::kan ^r nmt1*-cdc23-1E2-td::kan ^r leu1-32

this work

P1288

mcm7-GFP _{N 760} ::Tcs ::ura4 ⁺ ade6-M210 leu1-32 ura4-D18 h ^-

this work

P1292

ade6-M210 leu1-32 ura4-D18 h ^- [pREP3X-TEV-NLS(LEU2)]

this work

P1322

cdc23-CFP _{S 424} ::Tcs::ura4 ⁺ ade6-M210 leu1-32 ura4-D18 h ^-

this work

P1323-1

P1323-2

cdc23-CFP _{S 424} ::Tcs::ura4 ⁺ ade6-M210 leu1-32 ura4-D18 h ^- [pREP3X-TEV-NLS(LEU2)]

this work

P1376

cdc23-CFP _{S 424} ::Tcs::ura4 ⁺ leu1-32 ura4-D18 h ^- [pREP3X-TEV-NLS(LEU2)]

this work

P1409

cdc45(sna41)-YFP::ura4 ⁺ cdc23-CFP _{S 424} ::Tcs::ura4 ⁺ leu1-32 ura4-D18 ade6 [pREP3X-TEV-NLS(LEU2)]

this work

P1411

psf2-YFP::kan ^r ade6-M210 leu1-32 ura4-D18 h ^-

this work

P1453

psf2-YFP::kan ^r nmt1*-cdc23-1E2-td::kan ^r

this work

P1460

spp1-GFP::kan ^r cdc23-CFP _{S 424} ::Tcs::ura4 ⁺ leu1-32 ura4-D18 ade6 [pREP3X-TEV-NLS(LEU2)]

this work