Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z

Table 5 Doubling time of ts allele integrants

Strain	LEU2 locus	RT	37°C
YL13-01	TRZ1 WT	5.54 ± 0.26	1.44 ± 0.04
YL13-02	trz1-537	6.87 ± 0.85	2.88 ± 0.01
YL13-03	trz1-538	6.31 ± 1.03	4.87 ± 1.15
Strain	TRZ1 locus	RT	37°C
YPH499	WT	3.17	1.85
YL12-01	trz1-537	4.75 ± 0.06	3.67 ± 0.02

Yeasts were inoculated into 2 mls of SC-glucose medium and grew at 30°C overnight. The overnight cultures were diluted with SC-glucose medium to O.D. at 600 nm 0.1 and returned to 30°C with shaking. The O.D. 600 nm reading was monitored throughout the day until it was more 1. Doubling time was calculated as described in table 2.

ISSN: 1471-2199