| | Glucose | Sucrose |
|---|
| | RT | 30°C | 37°C | RT | 30°C | 37°C |
|---|
YPH499
| +++++ | ++++ | +++++ | ++++ | +++++ | +++++ |
YL09-01
| +++++ | ++++ | +++++ | ++++ | +++++ | +++++ |
YL10-01
| +++++ | +++ | +++ | ++ | ++++ | ++ |
YL10-02
| +++++ | +++ | +++ | ++ | ++++ | ++ |
YL10-03
| +++++ | ++++ | +++++ | ++++ | +++++ | +++++ |
YL03-47
| +++++ | ++++ | +++++ | ++++ | +++++ | +++++ |
| |
Raffinose
|
Galactose
|
| |
RT
|
30°C
|
37°C
|
RT
|
30°C
|
37°C
|
YPH499
| +++ | +++++ | +++ | ++ | +++++ | ++ |
YL09-01
| +++ | +++++ | +++ | ++ | +++++ | ++ |
YL10-01
| + | ++ | + | - | - | - |
YL10-02
| + | ++ | + | - | - | - |
YL10-03
| +++ | +++++ | +++ | ++ | +++++ | ++ |
YL03-47
| +++ | +++++ | +++ | ++ | +++++ | ++ |
- SC-glucose: 3 days at RT, 2 days at 30 and 37°C.
- SC-sucrose: 3 days at RT, 2 days at 30 and 37°C.
- SC-raffinose: 3 days at RT, 2 days at 30 and 37°C.
- SC-galactose: 4 days at RT, 2 days at 30 and 37°C.
- +++++ fully grown spot with pink color
- ++++ fully grown spot with weak pink color
- +++ fully grown spot with white color
- ++ clearly seen by eye
- + barely seen by eye
- Yeasts were inoculated into 2 mls of SC-raffinose medium and grew overnight at 30°C. The resultant overnight cultures were diluted with SC-raffinose medium to O.D. at 600 nm 0.2 and returned to growth at 30°C for another 2–4 hours to get to the exponential growth phase. Then the cultures were diluted to O.D. 600 nm 0.2. These freshly diluted cultures were serially diluted and spotted onto SC plate with different sugars added.
