The sequence and cleavage sites of PfPP7. Panel A: The complete sequence of P. falciparum PP7 is shown. Peptides coded for by alternate exons are in bold. The PfPPJ sequence is boxed. Antibodies PP7-1, -2 and -3 were made against synthetic peptides of the sequence HKVLIKTKKMLEENIK, EVIRETFEENQKLREK, EKNSLWNNLYKQDKDK, respectively (as shown) and used in immunoblot analysis in Fig. 7. The two proteolytic cleavage sites are indicated; one between D231 and M232 (closed triangle), and the other and less precise site within the STEG sequence (broken line). Note that the cleavage essentially produces PPJ from PP7. The two EF-hand motifs are boxed in both panels A and B. Panel B: Sequence homology between the three PP7 homologs of P. falciparum, Drosophila (RdgC) and human origin. The location of a 141-residue insert (not shown) in the Plasmodium sequence is indicated by the open triangle; it is absent in the other homologs.