Sequence similarity and cleavage sites of the plasmodial calcineurin B (CnB) subunit. The accession number of the CnB subunits are: P. falciparum (Pf) AAO33818, T. gondii (Tg) AAM97279, H. sapiens (Hs) NP_000936. The cleavage site is indicated by the closed triangle (between K32 and R33). The four EF-hand motifs are boxed. Antibodies were made against synthetic peptides of the sequence QKDLLQAANFSETDIK, GITKLASSTDDFQKKK and DMISHMDVGNKLKLE as shown, and used in immunoblot analysis in Fig. 4. Residues that contact CyP-CsA are asterisked [24, 25]. The % numbers at the end represent amino acid identities of the Plasmodium sequence with the others.