Figure 3
From: A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA replication
The association of XDbf4 with chromatin differs from that seen with XCdc7. (A) Xenopus sperm nuclei were incubated in metaphase or interphase extract for 30 min. Chromatin was isolated and immunoblotted with antibodies specific for XOrc1, XDbf4, XCdc7, XMcm7 and XCdc6. (B, C) Sperm nuclei were incubated at 10 ng/μl in interphase Xenopus egg extract. At the indicated times, (B) samples were assayed for DNA synthesis by [α-32P]dATP incorporation, or (C) chromatin was isolated and immunoblotted with antibodies specific for XOrc1, XCdc6, XMcm7, XDbf4 and XCdc7.