Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

Table 3 Recombinant formation by variant linear DNA species

Plasmid	Restriction digest	Recombining linear species	% Lac+
1019	Bam + Xho	T9ACB	27 ± 2
1019	Bam + Xho + Kpn	9ACB	0.6 ± 0.2
1047	Bam + Xho	T9ACB(Δχ)	27 ± 2
1052	Bam + Xho	TACB	14 ± 0.1
1055	Sph + Xho	GCB	95 ± 3
1056	BsrG + Xho	ACF	99 ± 0.5
1057	Bam + Xho	TCB	96 ± 2
1018	Bam + Xho	ACB	0.2 ± 0.04

The indicated DNAs were introduced into TP832 by electroporation. DNA segments are labeled as in Figures 3–5. B(Δχ) is a variant of the 1.3 kbp right-side lac flank from which the 396 bp immediately adjacent to the cat segment (C) have been deleted, eliminating a Chi site. Figures indicate the means and standard errors from two measurements. Numbers in the first row are from Figure 5, top left, and are repeated here for comparison.

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