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Table 3 Recombinant formation by variant linear DNA species

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

Plasmid Restriction digest Recombining linear species % Lac+
1019 Bam + Xho T9ACB 27 ± 2
1019 Bam + Xho + Kpn 9ACB 0.6 ± 0.2
1047 Bam + Xho T9ACB(Δχ) 27 ± 2
1052 Bam + Xho TACB 14 ± 0.1
1055 Sph + Xho GCB 95 ± 3
1056 BsrG + Xho ACF 99 ± 0.5
1057 Bam + Xho TCB 96 ± 2
1018 Bam + Xho ACB 0.2 ± 0.04
  1. The indicated DNAs were introduced into TP832 by electroporation. DNA segments are labeled as in Figures 3–5. B(Δχ) is a variant of the 1.3 kbp right-side lac flank from which the 396 bp immediately adjacent to the cat segment (C) have been deleted, eliminating a Chi site. Figures indicate the means and standard errors from two measurements. Numbers in the first row are from Figure 5, top left, and are repeated here for comparison.