Figure 8

PCR products indicating specific duplication junctions. Colonies of Lac+ recombinants were tested by PCR as described in the Methods section. Lanes labeled S are standards (1 kb ladder, Invitrogen). Lanes 1, 2, and 3 are type I, II, and III recombinants, respectively, formed by electroporation of cells with the lac::cat930 DNA species diagrammed in Figure 5. The sizes of the expected products are: type I-1272 bp, II-1026 bp, III-1872 bp. Lane 4 is a recombinant formed by electroporation of cells with the GCB DNA species diagrammed in Figure 7. The size of the expected product is 3032 bp. Lane 5 is a recombinant formed by electroporation of cells with the ACF DNA species diagrammed in Figure 7. The size of the expected product is 2765 bp. Lane 6 is a recombinant formed by infection of cells with λ lac::cat819. The expected size of the product is 3653 bp. Lanes 7–10 are wild type cells used as template in control PCRs with the primers used in lanes 1–3, 4, 5, and 6, respectively.